病毒学
冠状病毒
单克隆抗体
免疫荧光
免疫印迹
病毒
基因
生物
抗体
细胞培养
分子生物学
冠状病毒科
2019年冠状病毒病(COVID-19)
遗传学
传染病(医学专业)
医学
疾病
病理
作者
Fandan Meng,Jiechao Yin,Xunliang Li,Wei Yang,Guangxing Li,Xiaofeng Ren
出处
期刊:Hybridoma
[Mary Ann Liebert, Inc.]
日期:2010-08-01
卷期号:29 (4): 345-350
被引量:18
标识
DOI:10.1089/hyb.2010.0009
摘要
Transmissible gastroenteritis virus (TGEV) is a member of coronaviruses. The viral spike (S) protein mediates the interaction between TGEV and its susceptible cells. Here, we expressed a truncated gene encoding the N terminal half of TGEV S gene (designated S1 gene) in a prokaryotic system. The resulting S1 protein was used to immunize BALB/c mice followed by the generation of a monoclonal antibody (MAb). A generated MAb (7F9) was identified by ELISA and the chromosome number of the hybridoma cell was analyzed. The immunoreactivity of the MAb to TGEV S protein was confirmed by Western blot analysis. Moreover, immunofluorescence assays showed that the MAb is able to detect cell infection by TGEV. The MAb achieved in this study can be used as a specific diagnostic reagent for detecting TGEV S protein.
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