血桂碱
细胞凋亡
活性氧
MCF-7型
细胞生物学
癌细胞
程序性细胞死亡
细胞内
线粒体
生物
化学
癌症研究
癌症
生物化学
生物碱
人体乳房
植物
遗传学
作者
Ji-Young Lim,Yae-Lim Lee,Hae Rin Lee,Woo-Young Choi,Won-Ho Lee,Yung Hyun Choi
标识
DOI:10.5487/tr.2007.23.3.215
摘要
Although sanguinarine, a benzophenanthridine alkaloid, possesses anti-cancer properties against several cancer cell lines, the molecular mechanisms by which it inhibits cell growth and induces apoptosis have not been clearly understood. In order to further explore the critical events leading to apoptosis in sanguinarine-treated MCF-7 human breast carcinoma cells, the following effects of sanguinarine on components of the mitochondrial apoptotic pathway were examined: generation of reactive oxygen species (ROS), alteration of the mitochondrial membrane potential (MMP), and the expression changes of Bcl-2 family proteins. We show that sanguinarine-induced apoptosis is accompanied by the generation of intracellular ROS and disruption of MMP as well as an increase in pro-apoptotic Bax expression and a decrease of anti-apoptotic Bcl-2 and Bcl-xL expression. The quenching of ROS generation with N-acetyl-L-cysteine, the ROS scavenger, protected the sanguinarine-elicited ROS generation, mitochondrial dysfunction, modulation of Bcl-2 family proteins, and apoptosis. Based on these results, we propose that the cellular ROS generation plays a pivotal role in the initiation of sanguinarine-triggered apoptotic death.
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