适体
指数富集配体系统进化
化学
卟啉
毛细管电泳
DNA
离解(化学)
小分子
离解常数
组合化学
分子
色谱法
生物化学
分子生物学
有机化学
核糖核酸
受体
生物
基因
作者
Jing Yang,Michael T. Bowser
摘要
Capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX) has previously been used to select aptamers for large-molecule targets such as proteins, lipopolysaccharides, and peptides. For the first time, we have performed CE-SELEX selection for a small-molecule target, N-methyl mesoporphyrin (NMM), with a molecular weight of only 580 g/mol. DNA aptamers with high-nanomolar to low-micromolar dissociation constants were achieved after only three rounds of selection. This corresponds to an >50-fold improvement in affinity over the random library. Two out of eight randomly chosen aptamers were found to catalyze the metal insertion reaction of mesoporphyrin with 1.7- and 2.0-fold rate enhancements, respectively.
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