间充质干细胞
祖细胞
造血
骨髓
外周血单个核细胞
干细胞
细胞生物学
化学
免疫学
生物
男科
体外
医学
生物化学
作者
Nathalie Meuleman,Tatiana Tondreau,Alain Delforge,Marielle Dejeneffe,Martine Massy,Mark Libertalis,Dominique Bron,Laurence Lagneaux
标识
DOI:10.1111/j.1600-0609.2005.00611.x
摘要
Abstract: The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10–20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for their clinical use. Therefore, we have evaluated the capacity of expansion of MSC in a commercial serum‐free medium (UC) supplemented with a serum substitute (ULTROSER®) in comparison with a classical medium α ‐MEM containing 15% FBS. Bone marrow‐mononuclear cells collected from 12 volunteer healthy donors were expanded in two different culture media. MSCs isolated in the both media were morphologically similar and expressed identical phenotypic markers. After the primoculture (P0) and one passage, we obtained significantly more MSC and CFU‐F progenitors in UC medium than in α MEM. Their multipotentiality was preserved during culture, as well as their capacity to support haematopoiesis. In conclusion, our observations strongly suggest that UC is an optimal medium for ex vivo expansion of MSC: it allows a better cell expansion, preserves cell multipotentiality, reduces the culture period and contains low concentration of serum substitute. This medium seems suitable for clinical scale expansion of MSC.
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