奶油
环腺苷酸反应元件结合蛋白
CREB1号
信使核糖核酸
磷酸化
分子生物学
生物
转录因子
响应元素
细胞生物学
信号转导
抄写(语言学)
基因亚型
结合蛋白
基因表达
发起人
基因
生物化学
语言学
哲学
作者
Frank Müller,Peter Boknı́k,Andreas Horst,Jörg Knapp,Bettina Linck,Wilhelm Schmitz,Ute Vahlensieck,Michael Böhm,Mario C. Deng,Hans H. Scheld
出处
期刊:Circulation
[Ovid Technologies (Wolters Kluwer)]
日期:1995-10-15
卷期号:92 (8): 2041-2043
被引量:45
标识
DOI:10.1161/01.cir.92.8.2041
摘要
In end-stage failing human hearts and in rat hearts after prolonged in vivo beta-adrenergic treatment, several proteins involved in the cAMP-dependent signal transduction are altered on the protein, mRNA, or transcriptional level, eg, beta-adrenoceptors, G-proteins, or proteins of Ca2+ homeostasis. In many tissues, cAMP-dependent transcriptional regulation occurs through the cAMP response element binding protein (CREB) and related transcription factors binding as dimers to cAMP response elements (CREs) in the promoter regions of regulated genes.To investigate a possible role of CREB in the human heart, nuclear protein of explanted failing and nonfailing human hearts was used to test for CRE specific binding properties in gel mobility shift assays. CRE specific binding was found in competition studies, and CREB and its phosphorylated form were immunologically identified in supershift experiments. The alternatively spliced CREB isoforms CREB327 and CREB341 were found to be expressed on the mRNA level by the reverse transcriptase-polymerase chain reaction.We conclude that in the failing and nonfailing human heart, CREB is expressed on the protein and mRNA levels and that CREB is phosphorylated and able to bind to CREs, indicating a functional role of CREB in the human heart.
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