神经发生
亚颗粒带
室下区
双皮质醇
生物
齿状回
成纤维细胞生长因子
海马结构
溴脱氧尿苷
内分泌学
海马体
内科学
神经科学
细胞生物学
神经干细胞
干细胞
免疫学
医学
免疫组织化学
遗传学
受体
作者
Kunlin Jin,Yunjuan Sun,Lin Xie,Sophie Batteur,Xiao Ou Mao,Chris Smelick,Anna Logvinova,David A. Greenberg
出处
期刊:Aging Cell
[Wiley]
日期:2003-05-28
卷期号:2 (3): 175-183
被引量:379
标识
DOI:10.1046/j.1474-9728.2003.00046.x
摘要
Neurogenesis, which may contribute to the ability of the adult brain to function normally and adapt to disease, nevertheless declines with advancing age. Adult neurogenesis can be enhanced by administration of growth factors, but whether the aged brain remains responsive to these factors is unknown. We compared the effects of intracerebroventricular fibroblast growth factor (FGF)-2 and heparin-binding epidermal growth factor-like growth factor (HB-EGF) on neurogenesis in the hippocampal dentate subgranular zone (SGZ) and the subventricular zone (SVZ) of young adult (3-month) and aged (20-month) mice. Neurogenesis, measured by labelling with bromodeoxyuridine (BrdU) and by expression of doublecortin, was reduced by approximately 90% in SGZ and by approximately 50% in SVZ of aged mice. HB-EGF increased BrdU labelling in SGZ at 3 months by approximately 60% and at 20 months by approximately 450%, which increased the number of BrdU-labelled cells in SGZ of aged mice to approximately 25% of that in young adults. FGF-2 also stimulated BrdU labelling in SGZ, by approximately 25% at 3 months and by approximately 250% at 20 months, increasing the number of newborn neurones in older mice to approximately 20% of that in younger mice. In SVZ, HB-EGF and FGF-2 increased BrdU incorporation by approximately 140% at 3 months and approximately 170% at 20 months, so the number of BrdU-labelled cells was comparable in untreated 3-month-old and growth factor-treated 20-month-old mice. These results demonstrate that the aged brain retains the capacity to respond to exogenous growth factors with increased neurogenesis, which may have implications for the therapeutic potential of neurogenesis enhancement in age-associated neurological disorders.
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