红粉甲虫
生物
果蝇属(亚属)
胚胎
原位杂交
黑腹果蝇
基因
进化生物学
植物
动物
细胞生物学
遗传学
幼虫
基因表达
作者
Johannes B. Schinko,Nico Posnien,Sebastian Kittelmann,Nikolaus Koniszewski,Gregor Bucher
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2009-08-01
卷期号:2009 (8): pdb.prot5258-pdb.prot5258
被引量:52
摘要
INTRODUCTION The red flour beetle, Tribolium castaneum , has emerged as an important model system for studying the evolution of development. Studies with Tribolium complement the vast amount of research done with Drosophila . Developmental features that are conserved between Drosophila and Tribolium , such as body segmentation, are achieved by quite different means, and thus comparison of developmental mechanisms between these two insects can address many interesting questions concerning the evolution of morphology and other characters. Most in situ protocols used for Tribolium have been adapted from Drosophila studies. Whole-mount in situ hybridization is a standard technique to visualize the activity of genes in embryos. The single and double staining protocol presented here uses two nonfluorescent stains to reveal gene activity. The development of both stains can be monitored visually, allowing the strength of the signal to be adjusted as needed. Cells that express both of the genes under investigation are readily detected using a microscope. The use of EGTA during fixation increases the proportion of embryos that devitellinize upon methanol treatment.
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