Stability of #alpha#--chymotrypsin in different media

The activity and stability of α chymotrypsin in different media were studied. The influence of organic solvents, pH values, ionic strengths and ionic types on the stability of α chymotrypsin was preliminarily studied. The secondary structure of α chymotrypsin in different pH was studied by circular dichroism (CD) spectra. The results showed that α chymotrypsin could maintain its high activity and stability in some organic solvents for a long time. And the CD spectra of α chymotrypsin in solutions with different pH showed that in pH 10 0, the content of α helix and (or) β sheet of α chymotrypsin was higher than that in pH 5.0 or pH 7.0. The influence of ionic strengths on the activity and stability of α chymotrypsin was different in different pH values.