Study on inactivated enzyme methods of alliinase in garlic

In this paper the effects of four kinds of inactivated enzyme methods,including microwave,alcohol,microwave-methanol and water heating,on the alliinase activity of garlic were studied.RP-HPLC was used to determine alliin in samples.The results showed that microwave method's optimum conditions is 300 kW,3 min;alcohol method's optimum conditions is 90%(v/v),1:4(w/v) of material/water ratio,30 min;microwave-methanol combined method's optimum conditions is 400 kW,1 min;water heating method's optimum conditions is 90 ℃,40 min.