适体
检出限
化学
赭曲霉毒素A
滚动圆复制
生物传感器
底漆(化妆品)
胶体金
电化学
分析物
线性范围
电极
色谱法
表面等离子共振
纳米技术
核酸
DNA
材料科学
真菌毒素
分子生物学
生物化学
生物
DNA复制
物理化学
有机化学
食品科学
作者
Liwei Huang,Jingjing Wu,Lei Zheng,Haisheng Qian,Feng Xue,Yucheng Wu,Daodong Pan,Samuel B O Adeloju,Wei Chen
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2013-11-08
卷期号:85 (22): 10842-10849
被引量:109
摘要
A novel electrochemical aptasensor is described for rapid and ultrasensitive detection of ochratoxin A (OTA) based on signal enhancement with rolling circle amplification (RCA). The primer for RCA was designed to compose of a two-part sequence, one part of the aptamer sequence directed against OTA while the other part was complementary to the capture probe on the electrode surface. In the presence of target OTA, the primer, originally hybridized with the RCA padlock, is replaced to combine with OTA. This induces the inhibition of RCA and decreases the OTA sensing signal obtained with the electrochemical aptasensor. Under the optimized conditions, ultrasensitive detection of OTA was achieved with a limit of detection (LOD) of 0.065 ppt (pg/mL), which is much lower than previously reported. The electrochemical aptasensor was also successfully applied to the determination of OTA in wine samples. This ultrasensitive electrochemical aptasensor is of great practical importance in food safety and could be widely extended to the detection of other toxins by replacing the sequence of the recognition aptamer.
科研通智能强力驱动
Strongly Powered by AbleSci AI