[Effects of hypoxic preconditioning on the adhesion of neutrophils to vascular endothelial cells induced by hypoxia/reoxygenation].

To investigate whether hypoxic preconditioning (HPC) can inhibit neutrophils (PMN) adhesion to vascular endothelial cell (VEC).The adhesion of PMN to VEC was measured by endogenous enzyme hexosaminidase. The effect of HPC on the expression of endothelial cell (EC) adhesion molecules was determined with flow cytometry.After stimulated with 1 hour of hypoxia (H) followed by 4 hours of reoxygenation (R), VEC showed an enhanced PMN adhensivity in association with an increased surface expression of E-selectin and intracellular adhesion molecules-1 (ICAM-1). HPC suppressed the expression of E-selectin and ICAM-1 with a subsequent inhibition of PMN adhesion to hypoxia/reoxygenation (H/R) stimulated VEC.HPC inhibits PMN adhesion to VEC through regulating the expression of EC adhesion molecules.