Lonidamine-ethyl ester-mediated remodelling of the Sertoli cell cytoskeleton induces phosphorylation of plakoglobin and promotes its interaction with α-catenin at the blood–testis barrier

普氏球蛋白 支持细胞 细胞生物学 粘合连接 血睾丸屏障 生物 钙粘蛋白 化学 内科学 内分泌学 信号转导 精子发生 生物化学 连环素 细胞 Wnt信号通路 医学
作者
Dolores D. Mruk,Michele Bonanomi,Bruno Silvestrini
出处
期刊:Reproduction, Fertility and Development [CSIRO Publishing]
卷期号:29 (5): 998-998 被引量:7
标识
DOI:10.1071/rd15378
摘要

Several compounds affect male fertility by disrupting the adhesion of germ cells to Sertoli cells, which results in the release of undeveloped germ cells into the seminiferous tubule lumen that are incapable of fertilising the ovum. Indazole carboxylic acids are one class of compounds exhibiting such effects and they have been investigated as non-hormonal contraceptives for potential human use. The aims of this study were to investigate the effects of lonidamine-ethyl ester, an indazole carboxylic acid, on spermatogenesis and cell junctions, in particular, desmosomes. We found two doses of lonidamine-ethyl ester at 50 mg kg–1 to disrupt Sertoli–germ cell adhesion. By light and fluorescent microscopy, pronounced changes were observed in the distribution of actin microfilaments and intermediate filaments, as well as in the localisation of plakoglobin, a protein with structural and signalling roles at the desmosome and adherens junction at the blood–testis barrier. Furthermore, immunoblotting and immunoprecipitation experiments using testis lysates revealed a significant upregulation (P < 0.01) of plakoglobin and Tyr-phosphorylated plakoglobin. Co-immunoprecipitation experiments showed an increase in the interaction between plakoglobin and fyn proto-oncogene, an Src family non-receptor tyrosine kinase, after treatment, as well as an increase in the interaction between plakoglobin and α-catenin. Taken collectively, these data indicate that a disruption of Sertoli cell and spermatocyte–spermatid adhesion in the seminiferous epithelium by lonidamine-ethyl ester results in the phosphorylation of plakoglobin, thereby promoting its interaction with α-catenin at the blood–testis barrier.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
晓雯发布了新的文献求助10
1秒前
或习完成签到,获得积分20
1秒前
1秒前
完美世界应助小恶采纳,获得10
2秒前
北海_hello发布了新的文献求助10
4秒前
852应助科研通管家采纳,获得10
4秒前
酷波er应助科研通管家采纳,获得10
4秒前
搜集达人应助科研通管家采纳,获得10
4秒前
汉堡包应助科研通管家采纳,获得10
4秒前
Ava应助科研通管家采纳,获得10
4秒前
斯文败类应助科研通管家采纳,获得10
4秒前
NexusExplorer应助科研通管家采纳,获得10
4秒前
所所应助科研通管家采纳,获得10
4秒前
所所应助科研通管家采纳,获得10
4秒前
乐乐应助科研通管家采纳,获得10
4秒前
充电宝应助科研通管家采纳,获得10
4秒前
我是老大应助科研通管家采纳,获得10
5秒前
华仔应助科研通管家采纳,获得10
5秒前
科目三应助科研通管家采纳,获得10
5秒前
科研通AI2S应助科研通管家采纳,获得10
5秒前
李爱国应助于大本事采纳,获得10
5秒前
5秒前
5秒前
5秒前
5秒前
天玄一刀发布了新的文献求助10
5秒前
7秒前
自习室的幽灵完成签到,获得积分10
7秒前
FleurdelisDZhang完成签到,获得积分10
8秒前
深情安青应助感动的寒风采纳,获得10
10秒前
拓跋涵易完成签到,获得积分10
12秒前
12秒前
斯文败类应助鱼腩采纳,获得10
13秒前
秩枊发布了新的文献求助10
16秒前
一定长发布了新的文献求助10
16秒前
16秒前
充电宝应助小杨爱吃羊采纳,获得10
16秒前
不会仰泳的鱼完成签到,获得积分10
16秒前
传奇3应助呼呼夫人采纳,获得10
16秒前
高分求助中
Earth System Geophysics 1000
Studies on the inheritance of some characters in rice Oryza sativa L 600
Medicina di laboratorio. Logica e patologia clinica 600
《关于整治突出dupin问题的实施意见》(厅字〔2019〕52号) 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Language injustice and social equity in EMI policies in China 500
mTOR signalling in RPGR-associated Retinitis Pigmentosa 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3207040
求助须知:如何正确求助?哪些是违规求助? 2856445
关于积分的说明 8104758
捐赠科研通 2521574
什么是DOI,文献DOI怎么找? 1354842
科研通“疑难数据库(出版商)”最低求助积分说明 642071
邀请新用户注册赠送积分活动 613343