Structural Characterization of Ginsenosides from Flower Buds ofPanax ginsengby RRLC-Q-TOF MS

人参 人参皂甙 化学 四极飞行时间 质谱法 色谱法 传统医学 串联质谱法 医学 病理 替代医学
作者
Wei Wu,Ziyan Lu,Yaran Teng,Yingying Guo,Shuying Liu
出处
期刊:Journal of Chromatographic Science [Oxford University Press]
卷期号:: bmv115-bmv115 被引量:14
标识
DOI:10.1093/chromsci/bmv115
摘要

Ginseng flower bud as a part of Panax ginseng has received much attention as a valuable functional food with medicinal potential. A few studies focused on systematic and comprehensive studies on its major ingredients. This study aims to rapidly characterize ginsenosides in ginseng flower buds and provide scientific basis for developing functional food, exploiting pharmaceutical effects and making full use of ginseng resources. A rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method was developed for rapid qualitative and quantitative analysis of ginsenosides in ginseng flower buds. The compounds were identified by comparing retention time of the reference standards, accurate mass measurement and the fragment ions obtained from RRLC-Q-TOF-MS/MS analyses. A total of 14 kinds of ginsenosides were identified and 5 kinds of malonyl-ginsenosides were first tentatively identified in ginseng flower buds. Ten kinds of main ginsenosides were quantitatively analyzed. The developed RRLC-Q-TOF-MS method was demonstrated as an effective analytical means for rapid characterization of the ginsenosides in flower buds of P. ginseng. The research result is valuable for quality control, assessment of authenticity and stability evaluation of ginseng flower buds.
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