生物
布鲁氏菌病
病毒学
布鲁氏菌
内质网
液泡
布鲁氏菌
微生物学
细胞生物学
免疫学
细胞质
作者
Suzana P. Salcedo,Nicolas Chevrier,Thaís Lourdes Santos Lacerda,Amira Ben Amara,Sarah Gerart,Vilma Arce-Gorvel,Chantal de Chastellier,J.M. Blasco,Jean‐Louis Mège,Jean‐Pierre Gorvel
标识
DOI:10.1093/infdis/jit007
摘要
Brucellae replicate in a vacuole derived from the endoplasmic reticulum (ER) in epithelial cells, macrophages, and dendritic cells. In animals, trophoblasts are also key cellular targets where brucellae efficiently replicate in association with the ER. Therefore, we investigated the ability of Brucella spp. to infect human trophoblasts using both immortalized and primary trophoblasts. Brucella extensively proliferated within different subpopulations of trophoblasts, suggesting that they constitute an important niche in cases where the fetal-maternal barrier is breached. In extravillous trophoblasts (EVTs), B. abortus and B. suis replicated within single-membrane acidic lysosomal membrane-associated protein 1-positive inclusions, whereas B. melitensis replicated in the ER-derived compartment. Furthermore, B. melitensis but not B. abortus nor B. suis interfered with the invasive capacity of EVT-like cells in vitro. Because EVTs are essential for implantation during early stages of pregnancy, the nature of the replication niche may have a central role during Brucella-associated abortion in infected women.
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