重编程
神经科学
生物
逆行追踪
生物神经网络
绿色荧光蛋白
寄主(生物学)
细胞生物学
中枢神经系统
基因
生态学
生物化学
作者
Olof Torper,Daniella Rylander Ottosson,Maria Pereira,Shong Lau,Tiago Cardoso,Shane Grealish,Malin Parmar
出处
期刊:Cell Reports
[Elsevier]
日期:2015-07-01
卷期号:12 (3): 474-481
被引量:174
标识
DOI:10.1016/j.celrep.2015.06.040
摘要
Summary
The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain.
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