紧身衣
次氯酸
化学
荧光
流式细胞术
活性氧
生物分子
内生
生物物理学
试剂
检出限
生物化学
纳米技术
分子生物学
生物
材料科学
有机化学
色谱法
量子力学
物理
作者
Hao Zhu,Zhen Zhang,Saran Long,Jianjun Du,Jiangli Fan,Xiaojun Peng
出处
期刊:Nature Protocols
[Springer Nature]
日期:2018-09-24
卷期号:13 (10): 2348-2361
被引量:66
标识
DOI:10.1038/s41596-018-0041-6
摘要
Hypochlorous acid (HOCl) is a critical member of the reactive oxygen species (ROS) produced by immune cells to fight infections. On the other hand, HOCl in homeostasis causes oxidative damage to biomolecules and is linked to many diseases, including inflammatory, neurodegenerative, and cardiovascular diseases. Herein, we detail a procedure for the preparation of a boron-dipyrromethene (BODIPY)-derived fluorescent probe for HOCl (BClO) and its application as an imaging reagent in living cells. BClO is synthesized in one pot through a four-step procedure that is nearly the same as that for conventional BODIPY dye preparation, except for the ratio of starting materials. BClO has an extremely rapid response (saturated within seconds) and is ultrasensitive to HOCl. The detection limit of BClO reaches the subnanomolar range, which is the highest HOCl sensitivity to date. Taking advantage of the ultrasensitive character of BClO, we have previously demonstrated its ability to detect endogenous HOCl generated by macrophages and shown that it can also be used to discriminate cancer cell lines (which show high HOCl production) from non-cancer cell lines (which show low HOCl production). The protocol requires ~2 d for probe synthesis and up to ~18 h for fluorescence imaging and flow cytometry assays.
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