New cytotoxic indole-type alkaloids obtained from Rhazya stricta leaves

Seven alkaloids, of indole nucleus, were isolated from Rhazya stricta (Apocynaceae). Two of which are new, epi -rhazyaminine and 20- epi -sitsirikine. All isolated compounds were tested against three cancer cell lines; colon cancer (HCT-116), prostate cancer (PC-3), human hepatocellular liver carcinoma (HepG2) and normal adult African green monkey kidney (VERO). Seven alkaloids of indole nucleus were isolated from Rhazya stricta (Apocynaceae). Two of which are new epi -rhazyaminine ( 1 ) and 20- epi -sitsirikine ( 2 ), while the rest are known; eburenine ( 3 ), strictamine ( 4 ), (16 R )- E -isositsirikine ( 5 ), antirhine ( 6 ), and strictanol ( 7 ). The chemical structures of the isolated compounds were elucidated by interpretation of the obtained spectral data 1D ( 1 H, 13 C and DEPT) and 2D (COSY, HSQC, HMBC and NOESY) NMR, UV, IR and MS. Compounds 1 - 7 were tested against three cancer cell lines; human colorectal carcinoma (HCT-116), prostate cancer (PC-3), human hepatocellular liver carcinoma (HepG2) and normal adult African green monkey kidney (VERO). • , , , .