材料科学
膜
纳米颗粒
细菌细胞结构
抗菌活性
银纳米粒子
动作(物理)
金黄色葡萄球菌
抗菌剂
纳米技术
细菌
大肠杆菌
生物物理学
化学工程
作者
Denver P. Linklater,Vladimir A. Baulin,Xavier Le Guével,Jean‐Baptiste Fleury,Eric Hanssen,The Hong Phong Nguyen,Saulius Juodkazis,Gary Bryant,Russell J. Crawford,Paul Stoodley,Elena P. Ivanova
标识
DOI:10.1002/adma.202005679
摘要
Abstract It is commonly accepted that nanoparticles (NPs) can kill bacteria; however, the mechanism of antimicrobial action remains obscure for large NPs that cannot translocate the bacterial cell wall. It is demonstrated that the increase in membrane tension caused by the adsorption of NPs is responsible for mechanical deformation, leading to cell rupture and death. A biophysical model of the NP–membrane interactions is presented which suggests that adsorbed NPs cause membrane stretching and squeezing. This general phenomenon is demonstrated experimentally using both model membranes and Pseudomonas aeruginosa and Staphylococcus aureus , representing Gram‐positive and Gram‐negative bacteria. Hydrophilic and hydrophobic quasi‐spherical and star‐shaped gold (Au)NPs are synthesized to explore the antibacterial mechanism of non‐translocating AuNPs. Direct observation of nanoparticle‐induced membrane tension and squeezing is demonstrated using a custom‐designed microfluidic device, which relieves contraction of the model membrane surface area and eventual lipid bilayer collapse. Quasi‐spherical nanoparticles exhibit a greater bactericidal action due to a higher interactive affinity, resulting in greater membrane stretching and rupturing, corroborating the theoretical model. Electron microscopy techniques are used to characterize the NP–bacterial‐membrane interactions. This combination of experimental and theoretical results confirm the proposed mechanism of membrane‐tension‐induced (mechanical) killing of bacterial cells by non‐translocating NPs.
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