Rbm46, a novel germ cell-specific factor, modulates meiotic progression and spermatogenesis

生物 配子发生 生殖细胞 减数分裂 精子发生 斑马鱼 遗传学 细胞生物学 转录因子 基因 抄写(语言学) 分子生物学 胚胎发生 语言学 内分泌学 哲学
作者
Xiangyan Dai,Xinkai Cheng,Jianfei Huang,Yanping Gao,Deshou Wang,Zhi Wei Feng,Gang Zhai,Qiyong Lou,Jiangyan He,Zhijian Wang,Zhan Yin
出处
期刊:Biology of Reproduction [Oxford University Press]
卷期号:104 (5): 1139-1153 被引量:15
标识
DOI:10.1093/biolre/ioab016
摘要

Abstract It has been suggested that many novel RNA-binding proteins (RBPs) are required for gametogenesis, but the necessity of few of these proteins has been functionally verified. Here, we identified one RBP, Rbm46, and investigated its expression pattern and role in zebrafish reproduction. We found that rbm46 is maternally provided and specifically expressed in the germ cells of gonadal tissues using in situ hybridization, reverse transcription-PCR, and quantitative real-time polymerase chain reaction (qRT-PCR). Two independent rbm46 mutant zebrafish lines were generated via the transcription activator-like effector nuclease technique. Specific disruption of rbm46 resulted in masculinization and infertility in the mutants. Although the spermatogonia appeared grossly normal in the mutants, spermatogenesis was impaired, and meiosis events were not observed. The introduction of a tp53M214K mutation could not rescue the female-to-male sex-reversal phenotype, indicating that rbm46 acts independently of the p53-dependent apoptotic pathway. RNA sequencing and qRT-PCR subsequently indicated that Rbm46 might be involved in the posttranscriptional regulation of functional genes essential for germ cell development, such as nanos3, dazl, and sycp3, during gametogenesis. Together, our results reveal for the first time the crucial role of rbm46 in regulating germ cell development in vivo through promotion of germ cell progression through meiosis prophase I.
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