VON WILLEBRAND FACTOR IMMUNOHISTOCHEMICAL STAINING QUANTITATIVE OPTICAL DENSITY PARAMETERS IN THE ENDOTHELIUM AND FIBRINOID OF THE PLACENTA DURING SECUNDINES INFLAMMATION AND CONCOMITANT IRON DEFICIENCY ANEMIA IN GRAVIDAS.

The aim of the research was to set the optical density quantitative parameters of the von Willebrand factor immunohistochemical staining (vWF) in the endothelium and fibrinoid of the placenta during the secundines inflammation concomitant with iron deficiency anemia in gravidas. The total number of 198 placentas was studied. The immunohistochemical technique was performed using the visualization of the primary antibodies to vWF with a diaminobenzidine dye polymer system. The optical density of the histochemical staining was measured by means of computer microdensitometry after the digital copies of the images had been obtained. All the cases of the secundines inflammation and the structures under study were found to have a significant increase in the optical density of the vWF immunohistochemical staining in the endothelium of the blood vessels as compared to the physiological pregnancy. Iron deficiency anemia in gravidas (IDAG) contributes to an increase in the indices of the inflammation, the highest data pertaining to the endothelial cells of the placental basal plate in chronic basal deciduitis. The optical density of the staining in the fibrinoid of the chorionic and basal plates during chronic forms of chorioamnionitis and basal deciduitis is higher than the optical density inherent in physiological pregnancy. The intensity of staining increases in presence of all the forms of inflammation on the background of IDAG in comparison with physiological pregnancy with placenta inflammation. Compared with IDAG in absence of the inflammatory processes, only chronic inflammatory processes reveal a change in indices. Consequently, the optical density of the staining significantly increases in the endothelium of blood vessels in all forms of the secundines inflammation, in comparison with the physiological pregnancy, whereas in fibrinoid the same process is reported only in chronic course. In this case, IDAG is accompanied by maximum levels of optical density in the endothelium and fibrinoid, whereas in chronic, the average indices are higher than those in acute forms.