Short Communication: Optimazing culture and differentiation L6 cell, C2C12 cell and primary myoblast cells culture

Abstract. Lesmana R, Goenawan H, Tarawan VM, Setiawan I, Hidayat A, Supratman U. 2018. Short Communication: Optimizing culture and differentiation of L6 cell, C2C12 cell, and primary myoblast cells culture. Cell Biol Dev 2: 51-54. Alteration in skeletal muscle function and structure could result from many chronic diseases, drug treatment, or physical activity. Therefore, understanding the molecular mechanism of skeletal muscle diseases or the adaptation process will be very important and beneficial. In most cases, for example, during aging, the imbalance between anabolic and catabolic processes could initiate a decreased muscle mass and change its fiber structure. Unfortunately, there is no suitable animal model for study, and animal model development is time-consuming at some point. Next, it is good to study and elaborate a model on disease mechanisms, drug targets, and hormonal effects in skeletal muscle, which is not easy and tricky. Finally, the promising methods to study molecular biology properties of muscle are using in vitro systems utilizing 2 cell lines derived from mouse (C2C12 myoblast cells) and rat (L6 myoblast. Another establishment technique is using mixed and isolated single fiber primary myocytes culture.