The spontaneous location recognition task for assessing spatial pattern separation and memory across a delay in rats and mice

习惯化 任务(项目管理) 编码(内存) 分离(统计) 计算机科学 认知 相似性(几何) 分类 模式识别(心理学) 对象(语法) 工作记忆 神经科学 心理学 认知心理学 人工智能 空间记忆 机器学习 图像(数学) 经济 管理
作者
Amy C. Reichelt,Cecilia P. Kramar,Olivia R. Ghosh‐Swaby,Paul A. S. Sheppard,Brianne A. Kent,Pedro Bekinschtein,Lisa M. Saksida,Timothy J. Bussey
出处
期刊:Nature Protocols [Nature Portfolio]
卷期号:16 (12): 5616-5633 被引量:19
标识
DOI:10.1038/s41596-021-00627-w
摘要

Keeping similar memories distinct from one another is a critical cognitive process without which we would have difficulty functioning in everyday life. Memories are thought to be kept distinct through the computational mechanism of pattern separation, which reduces overlap between similar input patterns to amplify differences among stored representations. At the behavioral level, impaired pattern separation has been shown to contribute to memory deficits seen in neuropsychiatric and neurodegenerative diseases, including Alzheimer’s disease, and in normal aging. This protocol describes the use of the spontaneous location recognition (SLR) task in mice and rats to behaviorally assess spatial pattern separation ability. This two-phase spontaneous memory task assesses the extent to which animals can discriminate and remember object locations presented during the encoding phase. Using three configurations of the task, the similarity of the to-be-remembered locations can be parametrically manipulated by altering the spatial positions of objects—dissimilar, similar or extra similar—to vary the load on pattern separation. Unlike other pattern separation tasks, SLR varies the load on pattern separation during encoding, when pattern separation is thought to occur. Furthermore, SLR can be used in standard rodent behavioral facilities with basic expertise in rodent handling. The entire protocol takes ~20 d from habituation to testing of the animals on all three task configurations. By incorporating breaks between testing, and varying the objects used as landmarks, animals can be tested repeatedly, increasing experimental power by allowing for within-subjects manipulations. This two-phase task assesses the extent to which animals can discriminate and remember object locations. Unlike in similar tasks, the load on pattern separation during memory encoding—when pattern separation is thought to occur—varies.
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