化学
单糖
果糖
发酵
多糖
大小排阻色谱法
支化(高分子化学)
产量(工程)
色谱法
响应面法
生物化学
食品科学
有机化学
酶
材料科学
冶金
作者
Vasudev S. Wagh,Madhukar S. Said,Jagadish S. Bennale,Syed G. Dastager
标识
DOI:10.1016/j.carbpol.2022.119241
摘要
In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 × 104 Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of β-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation.
科研通智能强力驱动
Strongly Powered by AbleSci AI