哈卡特
基因沉默
基因敲除
RNA干扰
下调和上调
NF-κB
信号转导
细胞生物学
银屑病
生物
炎症
癌症研究
小干扰RNA
角质形成细胞
细胞凋亡
免疫学
核糖核酸
细胞培养
转染
基因
遗传学
作者
Zhenxian Yang,Xiran Yin,Cheng Chen,Shanying Huang,Xueqing Li,Jianjun Yan,Qing Sun
出处
期刊:Inflammation
[Springer Nature]
日期:2022-03-21
卷期号:45 (5): 1924-1935
被引量:8
标识
DOI:10.1007/s10753-022-01664-7
摘要
Psoriasis is a chronic inflammatory disease of the skin with a very complex pathogenesis. Circular RNAs (circRNAs) play important regulatory roles in many diseases, including psoriasis. In this study, we found that circOAS3 expression was significantly upregulated in both psoriatic tissues and M5-induced keratinocytes. Silencing circOAS3 in HaCaT and Ker-CT cells inhibited their viability, promoted apoptosis, and blocked the cell cycle from the G1 to the S phase. RNA pull-down and RNA immunoprecipitation (RIP) analyses led to the identification of a direct interaction between circOAS3 and heat shock cognate protein 70 (Hsc70). Silencing circOAS3 expression negatively influenced Hsc70 protein expression but not mRNA expression. circOAS3 knockdown suppressed the activation of the JNK/STAT3/NF-κB signaling pathway. circOAS3 or Hsc70 silencing led to downregulated protein IL-6 expression, thus reducing psoriatic inflammation in vitro. In conclusion, the interaction between circOAS3 and Hsc70 mediates the proliferation and psoriatic inflammation of HaCaT and Ker-CT cells through the JNK/STAT3/NF-κB signaling pathway, suggesting that circOAS3 or Hsc70 may be a promising therapeutic target for psoriasis.
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