圆锥角膜
磷蛋白
磷酸化
角膜上皮
蛋白质组学
串联质量标签
蛋白质组
化学
生物
癌症研究
计算生物学
细胞生物学
上皮
医学
生物化学
角膜
病理
定量蛋白质组学
基因
神经科学
作者
Amit Chatterjee,Vivek Ghose,Jankiraman Narayanan,Prema Padmanabhan,Gajanan Sathe,Sailaja Elchuri
出处
期刊:Proteomics
[Wiley]
日期:2022-07-01
卷期号:22 (18)
标识
DOI:10.1002/pmic.202100416
摘要
Keratoconus (KC) is non-inflammatory, bilateral progressive corneal ectasia, and a disease of established biomechanical instability. The etiology of KC is believed to be multifactorial. Although previous studies gained insight into the understanding of the disease, little is known thus far on global protein phosphorylation changes in keratoconus. We performed phosphoproteome analysis of corneal epithelium from control (N = 5) and KC patients. Tandem mass tag (TMT) multiplexing technology along with immobilized metal affinity chromatography (IMAC) were used for the phosphopeptides enrichment and quantitation. Enriched peptides were analyzed on Orbitrap Fusion Tribrid mass spectrometer. This leads to the identification of 2939 unique phosphopeptides derived from 1270 proteins. We observed significant differential phosphorylation of 591 phosphopeptides corresponding to 375 proteins. Our results provide first phosphoproteomic signature of the keratoconus disease and identified dysregulated signaling pathways that can be targeted for therapy in future studies.
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