类有机物
诱导多能干细胞
胚状体
神经科学
生物
人口
药物发现
阿尔茨海默病
干细胞
细胞生物学
病理
疾病
胚胎干细胞
医学
生物信息学
遗传学
基因
环境卫生
作者
Wenjuan Fan,Xudong Chen,Sun Yizheng,Shanshan Wu,Haili Wang,K. Shoma Suresh,Sasirekha Krishnan,Murugan Ramalingam,Jinbo Deng
出处
期刊:Journal of Biomaterials and Tissue Engineering
[American Scientific Publishers]
日期:2022-05-01
卷期号:12 (5): 888-896
标识
DOI:10.1166/jbt.2022.2991
摘要
Alzheimer’s disease (AD) is a progressive neurologic disorder that impacts a diverse population of older adults. As three-dimensional (3D) models are powerful tools for advancing AD studies, the authors have been developed AD cortical organoids to enable the observation of AD pathology at the cellular, tissue, and organ levels. For creating the model, APPSwe/Ind (APP) and PSEN1 (PS1) mutant genes were transfected into mouse induced pluripotent stem cells (iPSCs) following which the iPSC lines that expressed mutant APP and PS1 proteins were obtained. Then, using modified serum-free suspended embryoid body culture, AD cerebral organoids were made successfully at various ages. The AD model can show AD’s biochemical and pathological alterations, such as overexpressions of A β 40 and A β 42 and a decrease of GABAergic interneurons. The proposed model has the potential for implementation in many biomedical applications, including AD drug screening, stem cell transplant, and neuronal tissue engineering.
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