开枪
木质部
生物
拟南芥
染色体易位
互补
光合作用
膜转运
突变体
植物
运输机
拟南芥
细胞生物学
生物化学
生物物理学
作者
Su-Fang Meng,Bin Zhang,Ren-Jie Tang,Xiao-Jiang Zheng,Rui Chen,Cong-Ge Liu,Yan-Ping Jing,Hai-Man Ge,Chi Zhang,Yan-Li Chu,Ai-Gen Fu,Fu-Geng Zhao,Sheng Luan,Wen-Zhi Lan
标识
DOI:10.1016/j.molp.2022.01.011
摘要
ABSTRACT
Magnesium (Mg2+), as the essential structural component for chlorophyll, is absorbed from the soil by roots and transported to shoots to support photosynthesis in plants. However, the molecular mechanisms underlying root-to-shoot Mg2+ translocation remain largely unknown. We describe here the identification of four plasma membrane (PM)-localized transporters, named Mg2+ Release transporters (MGRs) that are critical for root-to-shoot Mg transport in Arabidopsis. Functional complementation assays in a Mg2+-uptake-deficient bacterial strain confirmed that these MGRs conduct Mg2+ transport. PM-localized MGRs (MGR4, 5, 6, and 7) were expressed primarily in root stellar cells and participated in the xylem loading step of the long-distance Mg2+ transport process. In particular, MGR4 and MGR6 played a major role in shoot Mg homeostasis, as their loss-of-function mutants became hypersensitive to low Mg2+ but were tolerant to high Mg2+ conditions. Reciprocal grafting analysis further demonstrated that MGR4 functions in the root to determine shoot Mg2+ accumulation and phenotypes under both low- and high-Mg2+ stress. Taken together, our study has defined the long-sought transporters responsible for root-to-shoot Mg transport in plants.
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