Continuous antibiotic attenuation in algal membrane photobioreactor: Performance and kinetics

The attenuation of 10 mixed antibiotics along with nutrients in a continuous flow mode by four freshwater microalgae (Haematococcus pluvialis, Selenastrum capricornutum, Scenedesmus quadricauda, and Chlorella vulgaris) was examined in membrane photobioreactors (MPBRs). At lab-scale, consistent removal of both antibiotic and nutrient was shown by H. pluvialis and S. quadricauda, respectively. The system exhibited better performance with enhanced removal at HRT 24 h compared to 12 h and 48 h. The highest removal efficiency of antibiotics was observed in H. pluvialis MPBR, with the mean antibiotic removal values of 53.57%- 96.33%. Biodegradation was the major removal pathway of the antibiotics in the algal-MPBR (AMPBR), while removal by bioadsorption, bioaccumulation, membrane rejection, and abiotic was minor. Then, the bacterial feature was studied and showed significant influence from system hydrodynamics. The kinetics of continuous flow antibiotic removal followed Stover-Kincannon and Grau second-order models, which revealed great potential of AMPBR to withstand antibiotic load. The latter coupled with the computational fluid dynamic simulation was successfully applied for the residual antibiotic prediction and potential system optimization. Overall, these results provide an important reference for continuous flow antibiotic removal using AMPBR.