适体
微泡
碳纳米管
化学
检出限
纳米技术
外体
CD63
材料科学
纳米颗粒
组合化学
色谱法
小RNA
分子生物学
生物化学
基因
生物
作者
Yaokun Xia,Mengmeng Liu,Liangliang Wang,Yan An,Wenhui He,Mei Chen,Jianming Lan,Jiaoxing Xu,Lunhui Guan,Jinghua Chen
标识
DOI:10.1016/j.bios.2017.01.063
摘要
Recently, many studies have shown the potential use of circulating exosomes as novel biomarkers for monitoring and predicting a number of complex diseases, including cancer. However, reliable and cost-effective detection of exosomes in routine clinical settings, still remain a difficult task, mainly due to the lack of adequately easy and fast assay platforms. Therefore, we demonstrate here the development of a visible and simple method for the detection of exosomes by integrating single-walled carbon nanotubes that being excellent water solubility (s-SWCNTs) and aptamer. Aptamers, specific to exosomes transmembrane protein CD63, are absorbed onto the surface of s-SWCNTs and improve the minic peroxidase activity of s-SWCNTs, which can efficiently catalyze H2O2-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and lead to a change from colorless to blue in solution. However, after adding exosomes, the aptamers are bound with CD63, leaving from the surface of s-SWCNTs through conformational changes, which results the color of solution from deep to moderate, and this can be observed by the naked eye and monitored by UV–vis spectrometry. Under optimal conditions, the linear range of exosomes is estimated to be 1.84×106 to 2.21×107 particles/μL with a detection of limit (LOD) of 5.2×105 particles/μL. Consequently, a visible and simple approach detecting exosomes is successfully constructed. Moreover, this proposed colorimetric aptasensor can be universally applicable for the detection of other targets by simple change the aptamer.
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