脂质代谢
脂滴
下调和上调
细胞器
代谢途径
细胞生物学
生物
生物化学
新陈代谢
化学
生物物理学
基因
作者
Yajuan Li,Phyllis Chang,Shriya Sankaran,Hongje Jang,Yuhang Nie,Audrey Zeng,Sahran Hussain,Jane Y. Wu,Xu Chen,Lingyan Shi
出处
期刊:GEN biotechnology
[Mary Ann Liebert]
日期:2023-06-01
卷期号:2 (3): 247-261
被引量:6
标识
DOI:10.1089/genbio.2023.0017
摘要
Studies have shown that brain lipid metabolism is associated with biological aging and influenced by dietary and genetic manipulations; however, the underlying mechanisms are elusive. High-resolution imaging techniques propose a novel and potent approach to understanding lipid metabolic dynamics in situ. Applying deuterium water (D2O) probing with stimulated Raman scattering (DO-SRS) microscopy, we revealed that lipid metabolic activity in Drosophila brain decreased with aging in a sex-dependent manner. Female flies showed an earlier occurrence of lipid turnover decrease than males. Dietary restriction (DR) and downregulation of insulin/IGF-1 signaling (IIS) pathway, two scenarios for lifespan extension, led to significant enhancements of brain lipid turnover in old flies. Combining SRS imaging with deuterated bioorthogonal probes (deuterated glucose and deuterated acetate), we discovered that, under DR treatment and downregulation of IIS pathway, brain metabolism shifted to use acetate as a major carbon source for lipid synthesis. For the first time, our study directly visualizes and quantifies spatiotemporal alterations of lipid turnover in Drosophila brain at the single organelle (lipid droplet) level. Our study not only demonstrates a new approach for studying brain lipid metabolic activity in situ but also illuminates the interconnection of aging, dietary, and genetic manipulations on brain lipid metabolic regulation.
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