杏鲍菇
化学
多糖
平菇
阿拉伯糖
鼠李糖
糖苷键
甘露糖
体外
半乳糖
木糖
生物化学
蘑菇
食品科学
酶
发酵
作者
Pin Gong,Hui Long,Yuxi Guo,Siyuan Wang,Fuxin Chen,Xuefeng Chen
出处
期刊:Molecules
[MDPI AG]
日期:2022-10-21
卷期号:27 (20): 7140-7140
被引量:20
标识
DOI:10.3390/molecules27207140
摘要
Pleurotus eryngii (PE) is an edible mushroom with high nutritional value. Pleurotus eryngii polysaccharides (PEPs) are one of the main active ingredients and manifest a great variety of biological activities. This study mainly focused on the chemical characterization and biological activities of PEPs, which were separated into two fractions (named WPS and P-1). WPS is mainly dominated by β-glycosidic bonds and contains α-glycosidic bonds, and P-1 only contains α-glycosidic bonds. The molecular weights of WPS and P-1 were 4.5 × 105 Da and 2.2 × 104 Da. The result of GC indicated that two the fractions were composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose, with a ratio of 0.35:0.24:0.45:0.24:28.78:1.10 for WPS and 0.95:0.64:0.66:1.84:60.69:0.67 for P-1. The advanced structure studies indicated that the two fractions had no triple-helical structure, where WPS had a dense structure and P-1 had a loose structure. In addition, the antioxidant activity of WPS surpassed P-1, and the two fractions also exhibited a high hypoglycemic activity via inhibiting α-glycosidase activities and promoting the expression of PI3K-AKT signaling pathway based on in vitro assay and cell experiments.
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