纳米载体
基因传递
绿色荧光蛋白
原生质体
荧光显微镜
化学
质粒
分子生物学
DNA
转染
共焦显微镜
生物物理学
报告基因
免疫印迹
生物
细胞生物学
基因表达
生物化学
荧光
基因
药物输送
物理
有机化学
量子力学
作者
Md Reyazul Islam,Marston Youngblood,Hye-In Kim,Ivonne González‐Gamboa,Andrea G. Monroy-Borrego,Adam A. Caparco,Gregory V. Lowry,Nicole F. Steinmetz,Juan Pablo Giraldo
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-06-18
卷期号:24 (26): 7833-7842
标识
DOI:10.1021/acs.nanolett.3c04735
摘要
Tobacco mild green mosaic virus (TMGMV)-like nanocarriers were designed for gene delivery to plant cells. High aspect ratio TMGMVs were coated with a polycationic biopolymer, poly(allylamine) hydrochloride (PAH), to generate highly charged nanomaterials (TMGMV-PAH; 56.20 ± 4.7 mV) that efficiently load (1:6 TMGMV:DNA mass ratio) and deliver single-stranded and plasmid DNA to plant cells. The TMGMV-PAH were taken up through energy-independent mechanisms in Arabidopsis protoplasts. TMGMV-PAH delivered a plasmid DNA encoding a green fluorescent protein (GFP) to the protoplast nucleus (70% viability), as evidenced by GFP expression using confocal microscopy and Western blot analysis. TMGMV-PAH were inactivated (iTMGMV-PAH) using UV cross-linking to prevent systemic infection in intact plants. Inactivated iTMGMV-PAH-mediated pDNA delivery and gene expression of GFP in vivo was determined using confocal microscopy and RT-qPCR. Virus-like nanocarrier-mediated gene delivery can act as a facile and biocompatible tool for advancing genetic engineering in plants.
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