自愈水凝胶
赫拉
磁性纳米粒子
化学
阿霉素
组织工程
纳米颗粒
MTT法
活力测定
纳米技术
生物物理学
生物医学工程
材料科学
细胞
生物化学
高分子化学
外科
生物
医学
化疗
作者
Basak Coban,Mehmet Başkurt,Hasan Şahin,Ahu Arslan Yıldız
标识
DOI:10.1002/mabi.202400339
摘要
Abstract Alginate forms a hydrogel via physical cross‐linking with divalent cations. In literature, Ca 2+ is mostly utilized due to strong interactions but additional procedures are required to disassociate Ca‐alginate hydrogels. On the other hand, Mg‐alginate hydrogels disassociate spontaneously, which might benefit certain applications. This study introduces Mg‐alginate as the main component of a bio‐ink for the first time to obtain 3D tumor models by magnetic bio‐patterning technique. The bio‐ink contains magnetic nanoparticles (MNPs) for magnetic manipulation, Mg‐alginate hydrogel as a sacrificial material, and cells. The applicability of the methodology is tested for the formation of 3D tumor models using HeLa, SaOS‐2, and SH‐SY5Y cells. Long‐term cultures are examined by Live/dead and MTT analysis and revealed high cell viability. Subsequently, Collagen and F‐actin expressions are observed successfully in 3D tumor models. Finally, the anti‐cancer drug Doxorubicin (DOX) effect is investigated on 3D tumor models, and IC 50 values is calculated to assess the drug response. As a result, significantly higher drug resistance is observed for bio‐patterned 3D tumor models up to tenfold compared to 2D control. Overall, Mg‐alginate hydrogel is successfully used to form bio‐patterned 3D tumor models, and the applicability of the model is shown effectively, especially as a drug screening platform.
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