适体
化学
检出限
石墨烯
指数富集配体系统进化
线性范围
荧光
乙酰甲胺磷
色谱法
氧化物
纳米技术
分子生物学
材料科学
生物化学
基因
物理
生物
核糖核酸
杀虫剂
有机化学
量子力学
农学
作者
Poonam Singh,Sandeep Kumar,Sanjay Kumar Verma
出处
期刊:Talanta
[Elsevier]
日期:2022-08-17
卷期号:252: 123843-123843
被引量:9
标识
DOI:10.1016/j.talanta.2022.123843
摘要
A fluorescent graphene oxide based aptasensing platform was developed for the detection of acephate. The aptamers specific to the acephate were screened through GO-SELEX (Graphene Oxide - Systematic Evolution of Ligands by EXponential enrichment) method for six rounds. The screened aptamers were analyzed for their binding affinity and specificity by using fluorescence-based assay. The aptamer AAPT3 that demonstrated highest affinity (Kd = 9 ± 1 nM) and an excellent selectivity, was employed in the development of fluorescent aptasensor. Under optimal conditions, the aptasensor showed low limit of detection (4 ng mL-1) and a wide dynamic linear range (5-80 ng mL-1). The aptasensor was also validated against water samples spiked with acephate, which showed fluorescence recovery from 94 to 107% and coefficient of variation 1-5%. These results indicate that the developed aptasensor can be used for sensitive, selective, and accurate detection of acephate in various samples.
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