盐度
多不饱和脂肪酸
小球藻
食品科学
化学
适应性
生物量(生态学)
植物
生物
生物化学
脂肪酸
藻类
农学
生态学
作者
Haoyang Li,Xin Sun,Youreng Sun,Lei Ye,Hanyu Xue,Feng Gao,Yu Yang
标识
DOI:10.1016/j.cej.2024.150409
摘要
Two-stage cultivation of Chlorella with periodic micro-current coupled with NaCl stress was investigated to analyze the performance and contribution of increased lipid production. In the growth stage, periodic micro-current of 3 mA resulted in an escalation of 290.06 mg/L of Chlorella's biomass by 61.25 % in comparison to the control. During the induction stage, under a NaCl concentration of 8 g/L, the highest lipid content and yield attained were 42.11 % and 199.32 mg/L, revealing an increment of 53.13 % and 39.11 % respectively when compared to the control. Additionally, the polyunsaturated fatty acids content (PUFA) decreased from 30.11 % to 23.09 %, while the saturated fatty acids content (SFA) increased from 31.86 % to 42.39 %. Chlorella demonstrated adaptability to the alterations in the cultivation environment by an initial upsurge and later a reduction in SOD and CAT enzyme activities, and concurrently facilitated lipid synthesis by similar changes in the activities of ACCase and DGAT. The above cultivation enabled a synergistic effect of swift growth and efficient lipid accumulation in Chlorella, providing groundwork for large-scale biofuel production.
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