Analysis of chemical composition and evaluation of antigenotoxic, cytotoxic and antioxidant activities of essential oil of Toddalia asiatica (L.) Lam

To analyse the chemical composition of essential oil isolated from the leaves of Toddalia asiatica (L.) Lam. and to test its bioactive properties. Gas chromatography-mass spectrometry (GC/MS) analysis and antigenotoxicity, cytotoxicity and antioxidant studies of isolated oil. GC-MS analysis of oil revealed the presence of 42 compounds. The major compound in the oil was β-phellandrene (21.35%). DNA protecting activity of oil was considerably significant against H2O2 induced genotoxicity in human lymphocytes. Concentration of oil needed to protect 50% of DNA was calculated as 100 μg/mL. Cytotoxicity of oil against breast (MCF-7) and colorectal (HT-29) cancer cells were observed with the IC50 value of (7.80±0.03) μg/mL and (100.00±0.16) μg/mL respectively. Considerable DPPH free radical, hydroxyl radical scavenging, iron chelation and inhibition of lipid peroxidation activities of oil were also studied. The results of the present study clearly indicate oil could be a promising candidate for food and drug preparation.