三角洲
基因
定量分析(化学)
计算生物学
实时聚合酶链反应
生物
样品(材料)
计算机科学
分子生物学
生物系统
遗传学
化学
色谱法
物理
天文
作者
Kenneth J. Livak,Thomas D. Schmittgen
出处
期刊:Methods
[Elsevier]
日期:2001-12-01
卷期号:25 (4): 402-408
被引量:153228
标识
DOI:10.1006/meth.2001.1262
摘要
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2−ΔΔCT method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2−ΔΔCT method. In addition, we present the derivation and applications of two variations of the 2−ΔΔCT method that may be useful in the analysis of real-time, quantitative PCR data.
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