荧光素酶
化学
磺胺
组合化学
报告基因
萤光素酶类
化学合成
酶
立体化学
生物化学
基因表达
基因
体外
转染
作者
Ali Nakhi,Md. Shafiqur Rahman,Ravada Kishore,Chandana Lakshmi T. Meda,Girdhar Singh Deora,Kishore V. L. Parsa,Manojit Pal
标识
DOI:10.1016/j.bmcl.2012.08.056
摘要
2-Substituted pyrrolo[2,3-b]quinoxalines having free NH were prepared directly from 3-alkynyl-2-chloroquinoxalines in a single pot by using readily available and inexpensive methane sulfonamide (or p-toluene sulfonamide) as an ammonia surrogate. The reaction proceeded in the presence of Cu(OAc)(2) affording the desired product in moderate yield. The crystal structure analysis of a representative compound and its supramolecular interactions are presented. Some of the compounds synthesized exhibited inhibitory activities against luciferase that was supported by the predictive binding mode of these compounds with luciferase enzyme through molecular docking studies. The key observations disclosed here can alert users of luciferase reporter gene assays for possible false positive results due to the direct inhibition of luciferase.
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