α-Calcium-Calmodulin-Dependent Kinase II is Associated with Paired Helical Filaments of Alzheimerʼs Disease

激酶 老年斑 化学 磷酸化 钙调蛋白 阿尔茨海默病 蛋白激酶A 细胞生物学 免疫金标记 蛋白激酶C 生物化学 生物 病理 医学 解剖 疾病 有机化学 超微结构
作者
Jinsong Xiao,George Perry,Juan C. Troncoso,Mervyn J. Monteiro
出处
期刊:Journal of Neuropathology and Experimental Neurology [Oxford University Press]
卷期号:55 (9): 954-963 被引量:44
标识
DOI:10.1097/00005072-199609000-00002
摘要

Alzheimer's disease (AD) is characterized pathologically by two distinguishable deposits in the brain, namely senile plaques and neurofibrillary tangles (NFT). Senile plaques are composed of fragments of the amyloid precursor protein, whereas NFT are composed primarily of paired-helical filaments (PHF). The latter are in turn composed principally of the microtubule-associated protein, tau. Tau in PHF is highly and unusually phosphorylated but the mechanisms leading to this unusual phosphorylation are not known. Using a combination of immunoblotting and kinase assays, we demonstrate that a discreet set of kinases copurify with PHF. One of these kinases was found by immunoblotting to be alpha-calcium-calmodulin-dependent kinase II (alpha-CaM kinase). Immunogold labeling revealed that alpha-CaM kinase was localized to a novel globular membranelike structure found at the ends of PHF. Since previous studies have shown alpha-CaM kinase to be involved in memory, its association with PHF may have important implications in understanding memory loss in AD. We also discuss the possibility that the association of alpha-CaM kinase with PHF may indicate sites where tau protein is converted into PHF.
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