氧化苦参碱
神经保护
NMDA受体
神经毒性
药理学
苦参
兴奋毒性
体内
谷氨酸受体
缺血
美金刚
化学
医学
海马结构
细胞凋亡
兴奋剂
受体
海马体
AMPA受体
生物
神经科学
生物化学
苦参碱
内科学
毒性
生物技术
作者
Kun Zhang,Yujiao Li,Qingqing Yang,Gerile Oudeng,Le Yang,Xubo Li,Yanyan Guo,Nan Zhang,Bin Feng,Shui-bing Liu,Ming Zhao
出处
期刊:Phytomedicine
[Elsevier BV]
日期:2013-02-01
卷期号:20 (3-4): 343-350
被引量:30
标识
DOI:10.1016/j.phymed.2012.10.018
摘要
Oxymatrine (OMT) is a major bioactive component derived from Sophora flavescens Ait (kushen), which is widely used in Chinese medicine. Recent studies have shown that it has neuroprotective effects; however, its underlying mechanisms remain unclear. We focus on the mechanisms of pharmacologic action in OMT by detecting its pharmacological properties against focal cerebral ischemia in vivo and NMDA-induced neurotoxicity in vitro. OMT prevented cerebral ischemic injury in mice induced via a 2 h middle cerebral artery occlusion and a 24 h reperfusion, in vivo. In vitro cultured neurons challenged with N-methyl-D-aspartate (NMDA, 200 μM) for 30 min showed significant decrease in the viability of neurons; however, OMT was able to protect neurons against induced neurotoxicity via NMDA exposure. Western blot analysis revealed that OMT decreased the expression of Bax and repaired the balance of pro- and anti-apoptotic proteins. Furthermore, OMT significantly reversed the up-regulation of NR2B and inhibited the calcium overload in the cultured neurons after challenging the NMDA. OMT showed partial protection in the cortical neurons via down-regulation of NR2B containing NMDA receptors and up-regulation of Bcl-2 family. Our results provide new insights into the development of natural therapeutic anti-oxidants against ischemia.
科研通智能强力驱动
Strongly Powered by AbleSci AI