相互作用体
生物发光
重要事件
蛋白质-蛋白质相互作用
蛋白质组学
能量转移
纳米技术
计算生物学
化学
生物
细胞生物学
生物化学
材料科学
地理
基因
考古
分子物理学
作者
Søren W. Gersting,Amelie S. Lotz‐Havla,Ania C. Muntau
出处
期刊:Methods in molecular biology
日期:2011-11-04
卷期号:: 253-263
被引量:13
标识
DOI:10.1007/978-1-61779-424-7_19
摘要
In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein–protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.
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