The OH* and CH* chemiluminescence in two-dimension, which were used to characterize the flame radiation, was measured in CH4 /O2 co-flowing jet diffusion flame by using hyperspectral and ultraviolet cameras. The results show that OH* and CH* chemiluminescence can reflect the structure of diffusion flame accurately. The distributions of OH* and CH* are different with equivalence ratios in laminar and turbulent flows. As the equivalent ratio increases in laminar flows, OH* distribution range gradually becomes large and extends to the downstream, while CH* mainly exists within the flame front that doesn't change with the equivalence ratios. But in turbulence, the effect of equivalent ratios on radicals' distribution range is reduced, the OH* and CH* peak intensity also changes with equivalent ratios.