A 3,7‐Dihydroxyphenoxazine‐based Fluorescent Probe for Selective Detection of Intracellular Hydrogen Peroxide

Abstract A novel N ‐borylbenzyloxycarbonyl‐3,7‐dihydroxyphenoxazine fluorescent probe (NBCD) for detecting H 2 O 2 in living cells is described. The probe could achieve high selectivity for detecting H 2 O 2 over other biological reactive oxygen species (ROS). In addition, upon addition of H 2 O 2 , NBCD exhibited color change from colorless to pink, which makes it a “naked‐eye” probe for H 2 O 2 detection. NBCD could not only be used to detect enzymatically generated H 2 O 2 but also to detect H 2 O 2 in living systems by using fluorescence spectroscopy, with a detection limit of 2 μ m . Importantly, NBCD enabled the visualization of epidermal growth factor (EGF)‐stimulated H 2 O 2 generation inside the cells.