Gut Microbiota Plays Essential Roles in Soyasaponin's Preventive Bioactivities against Steatohepatitis in the Methionine and Choline Deficient (MCD) Diet‐Induced Non‐Alcoholic Steatohepatitis (NASH) Mice

Scope Gut microbiota (GM) is involved in nonalcoholic steatohepatitis (NASH) development. Phytochemicals soyasaponins can prevent NASH possibly by modulating GM. This study aims to investigate the preventive bioactivities of soyasaponin monomers (SS‐A 1 and SS‐Bb) against NASH and explores the mechanisms by targeting GM. Methods and results Male C57BL/6 mice are fed with methionine and choline deficient (MCD) diet containing SS‐A 1 , SS‐Bb, or not for 16 weeks. Antibiotics‐treated pseudo germ‐free (PGF) mice are fed with MCD diet containing SS‐A 1 , SS‐Bb, or not for 8 weeks. GM is determined by 16S rRNA amplicon sequencing. Bile acids (BAs) are measured by UPLC‐MS/MS. In NASH mice, SS‐A 1 and SS‐Bb alleviate steatohepatitis and fibrosis, reduce ALT, AST, and LPS in serum, decrease TNF‐α, IL‐6, α‐SMA, triglycerides, and cholesterol in liver. SS‐A 1 and SS‐Bb decrease Firmicutes , Erysipelotrichaceae , unidentified‐Clostridiales , Eggerthellaceae , Atopobiaceae , Aerococcus , Jeotgalicoccus , Gemella , Rikenella , increase Proteobacteria , Verrucomicrobia , Akkermansiaceae , Romboutsia , and Roseburia . SS‐A 1 and SS‐Bb alter BAs composition in liver, serum, and feces, activate farnesoid X receptor (FXR) in liver and ileum, increase occludin and ZO‐1 in intestine. However, GM clearance abrogates the preventive bioactivities of SS‐A 1 and SS‐Bb against NASH. Conclusion GM plays essential roles in soyasaponin's preventive bioactivities against steatohepatitis in MCD diet‐induced NASH mice.