阿卡波糖
夹竹桃科
吲哚试验
植物化学
立体化学
对接(动物)
生物碱
化学
吲哚生物碱
α-淀粉酶
淀粉酶
IC50型
生物化学
生物
体外
酶
植物
医学
护理部
作者
Muhammad Tahir Muhammad,Mehdi A. Beniddir,Lacksany Phongphane,Mohamad Hafizi Abu Bakar,M. Hazwan Hussin,Khalijah Awang,Marc Litaudon,Unang Supratman,Mohamad Nurul Azmi
出处
期刊:Fitoterapia
[Elsevier]
日期:2024-04-01
卷期号:174: 105873-105873
被引量:1
标识
DOI:10.1016/j.fitote.2024.105873
摘要
Diabetes mellitus stands as a metabolic ailment marked by heightened blood glucose levels due to inadequate insulin secretion. The primary aims of this investigative inquiry encompassed the isolation of phytochemical components from the bark of Kopsia teoi, followed by the assessment of their α-amylase inhibition. The phytochemical composition of the K. teoi culminated in the discovery of a pair of new indole alkaloids; which are 16-epi-deacetylakuammiline N(4)-methylene chloride (akuammiline) (1), and N(1)-methoxycarbonyl-11-methoxy-12-hydroxy-Δ14–17-kopsinine (aspidofractinine) (2), together with five known compounds i.e. kopsiloscine G (aspidofractinine) (3), akuammidine (sarpagine) (4), leuconolam (aspidosperma) (5), N-methoxycarbonyl-12-methoxy-Δ16, 17-kopsinine (aspidofractinine) (6), and kopsininate (aspidofractinine) (7). All compounds were determined via spectroscopic analyses. The in vitro evaluation against α-amylase showed good inhibitory activities for compounds 5–7 with the inhibitory concentration (IC50) values of 21.7 ± 1.2, 34.1 ± 0.1, and 30.0 ± 0.8 μM, respectively compared with the reference acarbose (IC50 = 34.4 ± 0.1 μM). The molecular docking outputs underscored the binding interactions of compounds 5–7 ranging from −8.1 to −8.8 kcal/mol with the binding sites of α-amylase. Consequently, the outcomes highlighted the anti-hyperglycemic attributes of isolates from K. teoi.
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