A neural circuit for vocal production responds to viscerosensory input in the songbird

Motor performance is monitored continuously by specialized brain circuits and used adaptively to modify behavior on a moment-to-moment basis and over longer time periods. During vocal behaviors, such as singing in songbirds, internal evaluation of motor performance relies on sensory input from the auditory and vocal-respiratory systems. Sensory input from the auditory system to the motor system, often referred to as auditory feedback, has been well-studied in singing zebra finches ( Taeniopygia guttata), but little is known about how and where non-auditory sensory feedback is evaluated. Here we show that brief perturbations in air sac pressure cause short latency neural responses in the higher-order song control nucleus HVC (used as proper name), an area necessary for song learning and song production. Air sacs were briefly pressurized through a cannula in anesthetized or sedated adult male zebra finches, and neural responses were recorded in both nucleus paraambigualis (PAm), a brainstem inspiratory center, and HVC, a cortical premotor nucleus. These findings show that song control nuclei in the avian song system are sensitive to perturbations directly targeted to vocal-respiratory-or viscerosensory-afferents and support a role for multimodal sensory feedback integration in modifying and controlling vocal control circuits.