Fecal Microbiota Transplantation from Aged Mice Render Recipient Mice Resistant to MPTP-Induced Nigrostriatal Degeneration Via a Neurogenesis-Dependent but Inflammation-Independent Manner

MPTP公司 神经发生 肠道菌群 阿克曼西亚 双皮质醇 酪氨酸羟化酶 移植 神经炎症 多巴胺能 生物 炎症 内分泌学 内科学 帕金森病 多巴胺 免疫学 神经科学 医学 海马体 生物化学 疾病 齿状回 发酵 乳酸菌
作者
Chen-Meng Qiao,Yu Zhou,Wei Quan,Xiaoyu Ma,Liping Zhao,Yun Shi,Hui Hong,Jian Wu,Gu-Yu Niu,Y. Chen,Shaowei Zhu,Chun Cui,Wenjin Zhao,Yan‐Qin Shen
出处
期刊:Neurotherapeutics [Springer Science+Business Media]
卷期号:20 (5): 1405-1426 被引量:5
标识
DOI:10.1007/s13311-023-01420-1
摘要

Accumulating data support a crucial role of gut microbiota in Parkinson’s disease (PD). However, gut microbiota vary with age and, thus, will affect PD in an age-dependent, but unknown manner. We examined the effects of fecal microbiota transplantation (FMT) pretreatment, using fecal microbiota from young (7 weeks) or aged mice (23 months), on MPTP-induced PD model. Motor function, pathological changes, striatal neurotransmitters, neuroinflammation, gut inflammation and gut permeability were examined. Gut microbiota composition and metabolites, namely short-chain fatty acids (SCFAs), were analyzed. Neurogenesis was also evaluated by measuring the number of doublecortin-positive (DCX+) neurons and Ki67-positive (Ki67+) cells in the hippocampus. Expression of Cd133 mRNA, a cellular stemness marker, in the hippocampus was also examined. Mice who received FMT from young mice showed MPTP-induced motor dysfunction, and reduction of striatal dopamine (DA), dopaminergic neurons and striatal tyrosine hydroxylase (TH) levels. Interestingly and unexpectedly, mice that received FMT from aged mice showed recovery of motor function and rescue of dopaminergic neurons and striatal 5-hydroxytryptamine (5-HT), as well as decreased DA metabolism after MPTP challenge. Further, they showed improved metabolic profiling and a decreased amount of fecal SCFAs. High-throughput sequencing revealed that FMT remarkably reshaped the gut microbiota of recipient mice. For instance, levels of genus Akkermansia and Candidatus Saccharimonas were elevated in fecal samples of recipient mice receiving aged microbiota (AM + MPTP mice) than YM + MPTP mice. Intriguingly, both young microbiota and aged microbiota had no effect on neuroinflammation, gut inflammation or gut permeability. Notably, AM + MPTP mice showed a marked increase in DCX+ neurons, as well as Ki67+ cells and Cd133 expression in the hippocampal dentate gyrus (DG) compared to YM + MPTP mice. These results suggest that FMT from aged mice augments neurogenesis, improves motor function and restores dopaminergic neurons and neurotransmitters in PD model mice, possibly through increasing neurogenesis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
阿艺完成签到,获得积分10
刚刚
香蕉半邪完成签到,获得积分10
1秒前
futianyu完成签到 ,获得积分0
2秒前
Lavenda完成签到,获得积分10
2秒前
亮仔发布了新的文献求助10
3秒前
linhante完成签到 ,获得积分10
3秒前
背后的傥完成签到,获得积分10
4秒前
雪雪完成签到 ,获得积分10
5秒前
西红柿完成签到,获得积分0
6秒前
冷傲的迎南完成签到 ,获得积分10
6秒前
xiaoguang li完成签到,获得积分10
7秒前
8秒前
鹿c3完成签到,获得积分10
9秒前
10秒前
11秒前
千十一发布了新的文献求助10
12秒前
123完成签到,获得积分10
14秒前
15秒前
AJiE完成签到,获得积分10
16秒前
123发布了新的文献求助10
17秒前
朴实雨竹完成签到,获得积分10
18秒前
彭于晏应助快来和姐妹玩采纳,获得10
21秒前
布曲完成签到 ,获得积分10
23秒前
魏伯安发布了新的文献求助10
24秒前
zhiyu完成签到,获得积分10
25秒前
胡图图完成签到,获得积分10
26秒前
27秒前
夏夜完成签到 ,获得积分10
28秒前
彩色的德地完成签到,获得积分10
28秒前
Kevin应助无语采纳,获得20
29秒前
晨曦完成签到,获得积分10
30秒前
小雅完成签到 ,获得积分10
33秒前
34秒前
lqy完成签到,获得积分20
34秒前
exosome完成签到,获得积分10
38秒前
39秒前
草履虫完成签到,获得积分10
40秒前
Yuu完成签到,获得积分10
42秒前
雁塔完成签到 ,获得积分10
42秒前
任性的傲柏完成签到,获得积分10
43秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Neuromuscular and Electrodiagnostic Medicine Board Review 1000
こんなに痛いのにどうして「なんでもない」と医者にいわれてしまうのでしょうか 510
Textures of Liquid Crystals 500
The First Nuclear Era: The Life and Times of a Technological Fixer 500
岡本唐貴自伝的回想画集 500
Distinct Aggregation Behaviors and Rheological Responses of Two Terminally Functionalized Polyisoprenes with Different Quadruple Hydrogen Bonding Motifs 450
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3671400
求助须知:如何正确求助?哪些是违规求助? 3228186
关于积分的说明 9778945
捐赠科研通 2938498
什么是DOI,文献DOI怎么找? 1610051
邀请新用户注册赠送积分活动 760520
科研通“疑难数据库(出版商)”最低求助积分说明 736020