Image mosaicking is the process of geometrically aligning multiple images having an overlapping field-of-view to generate a high-resolution panoramic image with an expanded field-of-view. Mosaicking for biomedical image stitching is gaining interest as it can provide better visualization of the anatomical site and may assist the surgeons during minimally invasive surgery. While image mosaicking is an active research area for various potential clinical specializations, more work is necessary to realize biomedical image mosaicking as a clinically valuable tool, especially in surgery and interventions. This chapter gives an introduction to image mosaicking pipeline, motion models and different types of image mosaicking techniques. Clinical applications of image mosaicking are then provided, followed by current challenges hindering the clinical applicability of mosaicking.