银杏
银杏内酯
生物合成
茉莉酸
基因
银杏
茉莉酸甲酯
转录组
生物
机制(生物学)
基因家族
生物化学
化学
植物
基因表达
拟南芥
突变体
哲学
认识论
作者
Jinfa Du,Zhen Zhao,Wenbo Xu,Qiao-lei Wang,Ping Li,Xu Lu
出处
期刊:Tree Physiology
[Oxford University Press]
日期:2023-09-23
被引量:1
标识
DOI:10.1093/treephys/tpad121
摘要
Abstract Ginkgo biloba L., an ancient relict plant known as a ‘living fossil’, has a high medicinal and nutritional value in its kernels and leaves. Ginkgolides are unique diterpene lactone compounds in G. biloba, with favorable therapeutic effects on cardiovascular and cerebrovascular diseases. Thus, it is essential to study the biosynthesis and regulatory mechanism of ginkgolide, which will contribute to quality improvement and medication requirements. In this study, the regulatory roles of the JAZ gene family and GbCOI1/GbJAZs/GbMYC2 module in ginkgolide biosynthesis were explored based on genome and methyl jasmonate-induced transcriptome. Firstly, 18 JAZ proteins were identified from G. biloba, and the gene characteristics and expansion patterns along with evolutionary relationships of these GbJAZs were analyzed systematically. Expression patterns analysis indicated that most GbJAZs expressed highly in the fibrous root and were induced significantly by methyl jasmonate. Mechanistically, yeast two-hybrid assays suggested that GbJAZ3/11 interacted with both GbMYC2 and GbCOI1, and several GbJAZ proteins could form homodimers or heterodimers between the GbJAZ family. Moreover, GbMYC2 is directly bound to the G-box element in the promoter of GbLPS, to regulate the biosynthesis of ginkgolide. Collectively, these results systematically characterized the JAZ gene family in G. biloba and demonstrated that the GbCOI1/GbJAZs/GbMYC2 module could regulate ginkgolides biosynthesis, which provides a novel insight for studying the mechanism of JA regulating ginkgolide biosynthesis.
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