Optimizing E. coli as a formatotrophic platform for bioproduction via the reductive glycine pathway

of 22 in bioreactor fed-batch experiments, highlighting its potential use for industrial bioprocesses. Finally, demonstrating the strain's potential to support a sustainable, formate-based bioeconomy, lactate production from formate was engineered. The optimized strain generated 1.2 mM lactate -10% of the theoretical maximum- providing the first proof-of-concept application of the reductive glycine pathway for bioproduction.